Antibody（organelles, intracellular traffic）｜一般社団法人　日本細胞生物学会

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Antibody（organelles, intracellular traffic）

Antibody（organelles, intracellular traffic）

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
a-adaptin2011-08-29	AP.6	WB	IF	IHC	IP
	ABR		x100-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
AP-2 (ac)2011-08-29		WB	IF	IHC	IP
	Upstate

	rabbit
	Poly
	Hela細胞
a-adaptin2011-08-29	AC1-M11	WB	IF	IHC	IP
	ABR	x200

	mouse
	Mono, IgG2a
	Hela細胞
a-adaptin2011-08-29	clone 8	WB	IF	IHC	IP
	BD Transduction	x1000

	mouse
	Mono
	Hela細胞
g-adaptin 2011-08-29	88	WB	IF	IHC	IP
	BD Transduction		x200

	mouse	【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	saponin, mouse555がいい。 Better to use saponin and Alexa555 2nd antibody.
	Hela細胞
δ-adaptin2011-08-29	18	WB	IF	IHC	IP
	BD Transduction	x500	x200

	mouse
	mono	WBに使わないほうがいい、IFもSA4の方がきれい。 Not suitable for WB. For IF, antibody SA4 is better than this antibody.
	Hela細胞
g-adaptin 2011-08-29	M-18	WB	IF	IHC	IP
	Santa Cruz

	goat
	Poly
	Hela細胞
ATF62011-08-29	70B1413.1	WB	IF	IHC	IP
	Lifespan

	mouse
	Mono, IgG1,k
	Hela細胞
ADRP2011-08-29	AP125	WB	IF	IHC	IP
	PROGEN	x100	x20-50

	mouse	【PFA+Triton】: -OA:○, +OA:×,【PFA+Saponin】: -OA:○, +OA:△,【MeOH 】:×,【PFA+70%EtOH】: -OA:○, +OA:△【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
ARF2011-08-29	H-50	WB	IF	IHC	IP
	Santa Cruz	x200-1000

	rabbit
	Poly
	Hela細胞
ARF52011-08-29	M01	WB	IF	IHC	IP
	Abnova

	mouse
	Mono, IgG1
	Hela細胞
Arfaptin22011-08-29	2B5	WB	IF	IHC	IP
	Abnova	x1000	x200-500

	mouse	【PFA+Triton】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG2b
	Hela細胞
ARF2011-08-29	1D9	WB	IF	IHC	IP
	ABR	x500	x500

	mouse	【PFA+Triton】:×,【IS】:○？【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1
	Hela細胞
ARF12011-08-29	3F1	WB	IF	IHC	IP
	ABR	x200

	mouse
	mono, IgG2a
	Hela細胞
ARF32011-08-29		WB	IF	IHC	IP
	BD Transduction	x200

	mouse	【PFA+Triton】:×,【IS】:○？【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1
	Hela細胞
ARF42011-08-29		WB	IF	IHC	IP
	PTG

	rabbit
	Poly
	Hela細胞
ARF62011-08-29	3A-1	WB	IF	IHC	IP
	Santa Cruz	x2000	x200

	mouse	【PFA+Triton】:×,【IS】:○？,【TCA】:○【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG2b	IFはTCA固定 TCA fix is best for IF.
	Hela細胞
Arfaptin12011-08-29	I-19	WB	IF	IHC	IP
	Santa Cruz	x1000	x100

	goat	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	WB:バンドが二本 Two bands appear by WB.
	Hela細胞
Arfaptin12011-08-29	N-20	WB	IF	IHC	IP
	Santa Cruz	x1000	x100

	goat	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	WB:バンドが一本、お勧め A unique band appear by WB. Recommended Ab.
	Hela細胞
Arfaptin22011-08-29		WB	IF	IHC	IP
	Zymed	x1000	x100

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
ATGL2011-08-29		WB	IF	IHC	IP
	Cayman		x100

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【MeOH 】:×,【PFA+70%EtOH 】:×,【PFA_digitonin】:△(Can Get B)【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS【digitonin条件】50ug/ml digitonin /PBS
	Poly
	Hela細胞
Atg122011-09-27		WB	IF	IHC	IP
	Cell Signaling	1:1000	1:100
	#2011
	rabbit
	p	mouse specific
	NIH3T3
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	4%PFA/PBS
	rabbit

	mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	mouse oocyte
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	4%PFA/PBS
	rabbit

	HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	HeLa
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	MEF
phospho-Aurora A(T288) B(T232) C(T198)2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2914	ice cold Methanol
	rabbit

	NIH3T3
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	HeLa
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	MEF
phospho-AuroraA/AIK (T288)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3091	ice cold Methanol
	rabbit

	NIH3T3
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	HeLa
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	MEF
AuroraA/AIK2014-06-04		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#3092	ice cold Methanol
	rabbit

	NIH3T3
alkaline phosphatase2013-07-04		WB	IF	IHC	IP
	Rockland		x100
	200-4135	3%PFA (in PBS)
	rabbit	0.05%saponin
		apical staining
	Caco2

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
alkaline phosphatase2013-12-04		WB	IF	IHC	IP
	Rockland		x100
	200-4135	3%PFA (in PBS)
	rabbit	0.05%saponin

	MDCK

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
actin beta2016-11-21		WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	251003	4% PFA
	Rabbit
	IgG
	HeLa, PC12
APPL12016-11-21		WB	IF	IHC	IP
	Cell signaling	1/1000	1/500
	3858	4% PFA
	Rabbit
	IgG
	HeLa, PC12
Alkaline Phosphatase2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (MitoSciences)
	459260
	Mouse
	monoclonal

phospho-AuroraB (T232)2014-07-11		WB	IF	IHC	IP
	ROCKLAND		x400
	600-401-677	4%PFA/PBS
	rabbit

	HeLa
Annexin II2017-10-10	5/Annexin II	WB	IF	IHC	IP
	BD		1/100
	610068	4% PFA at 4oC over night
	Mouse	0.5% Triton in PBS
	IgG1
	zebrafish embryos (8-10 hpf/hours post fertilization)
adaptin-delta2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610328	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining
	Caco2細胞
adaptin beta2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	610381	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
adaptin-beta2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610381	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining (similar to alpha)
	Caco2細胞
adaptin-beta2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610381	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining (similar to alpha)
	MDCK細胞

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
adaptin beta2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610381	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
adaptin gamma2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	610385	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
adaptin-gamma2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610385	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining (larger than alpha)
	Caco2細胞
adaptin-gamma2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610385	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining (larger than alpha)
	MDCK細胞

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
adaptin gamma2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610385	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
ap1802013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610469	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	included in Coated vesicle sampler kit
	Caco2細胞

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
AP1802014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610469	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
adaptin alpha2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	610501	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
adaptin-alpha2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610501	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining
	Caco2細胞
adaptin-alpha2013-03-18		WB	IF	IHC	IP
	BD transduction		x100
	610501	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining
	MDCK細胞

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
adaptin alpha2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610501	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
rab42013-07-04		WB	IF	IHC	IP
	BD transduction		x100
	610888	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining like adaptin alpha , included in Coated vesicle sampler kit
	Caco2細胞
g-adaptin 2011-08-29	100.3	WB	IF	IHC	IP
	SIGMA	x500-1000	x200
	A4200
	mouse	【PFA+Triton】:◎,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2b	MDCKII OK。Triton, mouse488がいい。 Alexa488 anti-mouse antibody is suitable for 2nd Ab.
	Hela細胞
a-adaptin2011-08-29	100/2	WB	IF	IHC	IP
	SIGMA	x200	―
	A4325
	mouse
	Mono, IgG2a
	Hela細胞
b-adaptin2011-08-29	100/1	WB	IF	IHC	IP
	SIGMA	x200	x100
	A4450
	mouse
	Mono, IgG1
	Hela細胞
ASAP12011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab64185
	rabbit
	IgG
	Hela細胞
AP-22011-08-14		WB	IF	IHC	IP
	CALBIOCHEM
	CP46
	mouse
	m

mouse adipophilin/ADRP2011-08-10		WB	IF	IHC	IP
	Progen		x100
	GP40	3%PFA (in PBS)
	guinea pig
	p
	マウス肝臓
Atg16L2011-09-27		WB	IF	IHC	IP
	MBL	1:1000
	PM040
	rabbit
	p	WBのみ使用可能

human ARF62011-08-10	3A-1	WB	IF	IHC	IP
	Santa Cruz		x100
	sc7971	3%PFA (in PBS), methanol
	mouse
	m
	マウス小腸
APN2013-07-04		WB	IF	IHC	IP
	BMA Biomedicals		x100
	T-2015	3%PFA (in PBS)
	rat	0.05%saponin
	IgG2A
	Caco2

【a】

【A】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
APN2013-12-04		WB	IF	IHC	IP
	BMA Biomedicals		x100
	T-2015	3%PFA (in PBS)
	rat	0.05%saponin
	IgG2A
	MDCK

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
acetylated-tubulin2012-09-14	6-11B-1	WB	IF	IHC	IP
	Sigma		x1000
	T7451	3%PFA in PBS
	mouse	0.1%Triton
	IgG2b	線毛を見るには、1次抗体は1%NGS, 0.1%Triton　4度overnightで染色する必要あり！！（0.05% saponinだと細胞内の微小管が多く染まるため不適） To visualize cilia, use 1st Ab in 1%NGS, 0.1%Triton overnight 4C, BG very high by 0.05% saponin.
	MEF

【B】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Beclin 12011-09-27		WB	IF	IHC	IP
	Cell Signaling	1:1000
	#3738
	rabbit
	p

BiP2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	G73320	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG2a
	MDCK細胞
BiP2012-09-14		WB	IF	IHC	IP
	transduction lab		x100
	G73320	3%PFA in PBS
	mouse	0.05% saponin
	IgG2a
	Caco2

【b】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
BiP(GRP78)2014-02-04		WB	IF	IHC	IP
	ABR		x100
	PA1-014	3%PFA (in PBS)
	rabbit	0.05%saponin

	MDCK

【B】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Beclin 12011-09-27		WB	IF	IHC	IP
	MBL	1:1000
	PD017
	rabbit
	p

【C】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
COPII2011-08-29		WB	IF	IHC	IP
	ABR		検出できず

	rabbit
	Poly
	Hela細胞
ε-COP2011-08-29		WB	IF	IHC	IP
	Santa Cruz	x200

	rabbit
	Poly
	Hela細胞
β'-COP (TCP-1)2011-08-29		WB	IF	IHC	IP
	StressGen		x200

	rat
	mono	MDCKII OK。
	Hela細胞
β'-COP2011-08-29		WB	IF	IHC	IP
	ABR	x200

	rabbit
	Poly
	Hela細胞
Calnexin2011-08-29		WB	IF	IHC	IP
	Stress Gen	x1000	x200

	rabbit	【PFA+Triton】:×,【MeOH】:△,【TCA】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Poly
	Hela細胞
α-COP2011-08-29		WB	IF	IHC	IP
	ABR	x200

	rabbit
	Poly
	Hela細胞
b-COP2011-08-29	PA1-061	WB	IF	IHC	IP
	ABR	x1000	x200x500

	rabbit
	Poly	Ptk2 ダメ、MDCKII OK。 Not suitable for PtK2.
	Hela細胞
Cathepsin D2011-08-29	Ab-2	WB	IF	IHC	IP
	CALBIOCHEM		x20

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:◎,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	Lamp1, LBPAと共局在する。 Colocalize with lamp1 and LBPA.
	Hela細胞
Cathepsin D2011-08-29		WB	IF	IHC	IP
	CALBIOCHEM		x2000

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:◎,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	Lamp1, LBPAと共局在する。 Colocalize with lamp1 and LBPA.
	Hela細胞
Clathrin2011-08-29	CHC5.9	WB	IF	IHC	IP
	PROGEN

	mouse
	Mono, IgM	IgM
	Hela細胞
Caveolin2011-08-29	clone 2297	WB	IF	IHC	IP
	BD Transduction		x100-

	mouse	【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	染まり方にムラがある。 Uneven staining
	Hela細胞
Clathrin2011-08-29	X22	WB	IF	IHC	IP
	ABR	x500	x1000x500

	mouse	【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	HeLaではだまが見える Aggregates are observed in HeLa cells.
	Hela細胞
CHC2011-08-29	clone 23	WB	IF	IHC	IP
	BD Transduction	x1000

	mouse
	Mono, IgG1
	Hela細胞
CHC2011-08-29	TD.1	WB	IF	IHC	IP
	Santa Cruz	x1000	x500

	mouse
	Mono, IgG1	WB感度よくない Not sensitive for WB.
	Hela細胞
CD632011-08-29		WB	IF	IHC	IP
	Ancell		x1000

	mouse	【PFA+Triton】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1
	Hela細胞
CI-MPR2013-01-19	86f7	WB	IF	IHC	IP
	DSHB		x100
		3%PFA (in PBS)
	mouse	0.05%saponin
	IgG
	Caco2細胞
CI-MPR2013-01-19	86f7	WB	IF	IHC	IP
	DSHB		x100
		3%PFA (in PBS)
	mouse	0.05%saponin
	IgG
	MDCK細胞
CD-MPR 2013-01-19	22d4	WB	IF	IHC	IP
	DSHB		x100
		3%PFA (in PBS)
	mouse	0.05%saponin
	IgG
	Caco2細胞
CD-MPR 2013-01-19	22d4	WB	IF	IHC	IP
	DSHB		x100
		3%PFA (in PBS)
	mouse	0.05%saponin
	IgG
	MDCK細胞
cathepsin B2011-09-22		WB	IF	IHC	IP
	Upstate		1/100-1/500
	06-480
	rabbit	4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
	poly
	mouse E6.5 embryo.MEF
rat cellubrevin2011-08-10	VAMP-3	WB	IF	IHC	IP
	SynapticSystems	x1000
	104102
	rabbit
	p
	マウス小腸、腎臓、脳
rat cellubrevin2011-08-10	VAMP-3	WB	IF	IHC	IP
	Synaptic Sy	x1000
	104102
	rabbit
	p
	マウス
Caveolin2011-10-17		WB	IF	IHC	IP
	BD Transduction	X1000	X500
	610059
	rabbit	【3.7% formalin solution +0.5%Triton】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	Poly	ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	HeLa細胞
clathrin heavy chain2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	610499	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
clathrin heavy chain2013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610499	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining , included in Coated vesicle sampler kit
	Caco2細胞
clathrin heavy chain2013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610499	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining , included in Coated vesicle sampler kit
	MDCK細胞
calnexin2011-09-27		WB	IF	IHC	IP
	BD Biosciences		1:100
	610523
	mouse
	m
	HeLa, A549, MCF7他
CPY (carboxypeptidase Y)2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (MitoSciences)
	A-6428
	Mouse
	monoclonal

Calnexin2011-10-04 About Product		WB	IF	IHC	IP
	Abcam		X100
	ab13504
	rabbit	【4%PFA+0.2%Saponin】:○

	HL-60細胞
Clathrin HC2011-10-06 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam		x400
	ab2731
	mouse	Methanol: ○
	IgG1
	NIH3T3
mouse cathepsin D2011-08-10		WB	IF	IHC	IP
	R&D Systems		x100
	AF1029	3%PFA (in PBS)
	goat

	マウスMEF

【c】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
cathepsin D2014-02-04		WB	IF	IHC	IP
	R&D systems		x100
	AF1029	3%PFA (in PBS)
	rabbit	0.05%saponin

	MDCK

【C】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Calnexin2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	C45520	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
calnexin2012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	C45520	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
calnexin2012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	C45520	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
calnexin2012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	C45520	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	殆どの細胞で核膜は染まる。染まる細胞と染まらない細胞の差が大きい Nuclear envelopes are stained in most cells. High variation in staining intensities among cells.
	Caco2細胞
calnexin2012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	C45520	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Cytoplasmic dynein2011-10-06	70.1	WB	IF	IHC	IP
	Sigma		x400
	D5167
	mouse	4%PFA+0.1%Saponin :○
	IgM
	HeLa
b-COP2011-08-29	M3A5	WB	IF	IHC	IP
	SIGMA	x50	x20
	G2279
	mouse
	Mono, IgG1
	Hela細胞
b-COP2011-08-29	MaD	WB	IF	IHC	IP
	SIGMA	x1000	x80?
	G6160
	mouse
	Mono, IgG1
	Hela細胞
b-COP2011-08-29	MaD	WB	IF	IHC	IP
	SIGMA	x1000	x500
	G6160
	mouse	【PFA+Triton】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1	IFの時、0.1% Triton+0.05%SDSを使う。 For IF, use 0.1% Triton+0.05%SDS
	Hela細胞
CathepsinD2011-09-27		WB	IF	IHC	IP
	Biodesign	1:1000	1:500
	K5016R
	rabbit
	p
	HeLa, A549, MCF7他
chicken IgYαCert2011-08-10		WB	IF	IHC	IP
	genway		x100
	m-150	3%PFA (in PBS)
	chicken
	p
	マウス小腸
human clathrin heavy chain2011-08-10		WB	IF	IHC	IP
	Affinity BioR		x100
	MA1065	3%PFA (in PBS)
	mouse
	m
	マウス小腸
human clathrin heavy chain2011-08-12		WB	IF	IHC	IP
	Affinity BioR
	MA1065
	mouse
	m	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

human clathrin heavy chain2012-08-03		WB	IF	IHC	IP
	Affinity BioR		x100
	MA1065	3%PFA in PBS
	mouse	0.05% saponin

	PC12
L12012-08-03		WB	IF	IHC	IP
	Chemicon		x100
	MAB5272	3%PFA in PBS
	rat	0.05% saponin

	PC12
caveolin-12011-08-10	N-20	WB	IF	IHC	IP
	Santa Cruz		x100
	sc894	3%PFA (in PBS)
	rabbit
	p	平滑筋が染まる Stains smooth muscles
	マウス小腸
calreticulin2012-03-02		WB	IF	IHC	IP
	Stressgen		x100
	SPA-600	methanol
	rabbit
		3%PFA in PBSでも染まるが弱い Only weakly stained by 3%PFA in PBS fixation
	mouse embryonic fibroblast (MEF)
rat chromogranin A2011-08-10		WB	IF	IHC	IP
	Yanaihara		x100
	Y291	3%PFA (in PBS)
	rabbit
		neuroendocrine cellsが染まる This Ab stains neuroendocrine cells.
	マウス小腸

【D】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
delta SA42011-08-10	AP-3	WB	IF	IHC	IP
	DSHB

	mouse
	m	試してない？ Not used

Dynamin II2011-08-29	27	WB	IF	IHC	IP
	BD Transdution	x250

	mouse
	Mono, IgG2a
	Hela細胞
Dynamin2011-08-29	41	WB	IF	IHC	IP
	BD Transdution	x1000

	mouse
	Mono, IgG1
	Hela細胞
Dynein heavy chain2011-08-29		WB	IF	IHC	IP
	トランスジェニック Trans Genic Inc.

	rabbit
	Poly
	Hela細胞
Dpm1 (Dolichol phosphate mannose synthase)2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (Molecular Probes)
	A-6429
	Mouse
	monoclonal

Dysbindin2011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	ダメ?	ダメ
	ab54548
	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	IgG
	Hela細胞
DPP IV2013-07-04		WB	IF	IHC	IP
	R&D systems		x100
	AF954	3%PFA (in PBS)
	goat	0.05%saponin
		apical staining
	Caco2

【d】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
DPP IV2013-12-04		WB	IF	IHC	IP
	R&D systems		x100
	AF954	3%PFA (in PBS)
	goat	0.05%saponin

	MDCK

【D】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Dynein intermediate chain2011-08-29	70/1	WB	IF	IHC	IP
	Sigma
	D5167
	mouse
	Mono
	Hela細胞
dynamin II2011-10-06		WB	IF	IHC	IP
	Thermo scientific		x400
	PA1-661
	Rabbit	Methanol: ○
	polyclonal
	NIH3T3

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
EEA12011-08-29	PA1-063	WB	IF	IHC	IP
	ABR	x1000	x250

	rabbit
	Poly	EEA1(mono)とパターン違う。 Staininig pattern is different from the one by EEA1 monoclonal Ab.
	Hela細胞
EEA12011-08-29	N-19(sc-6415)	WB	IF	IHC	IP
	Santa Cruz		x30

	goat	【PFA+Triton】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Poly	IFでBGあるが使える Some background staining appears in IF staininig.
	Hela細胞
EEA12011-08-29	C-15(sc-6414)	WB	IF	IHC	IP
	Santa Cruz		ダメ?

	goat
	Poly	IFで使えない。 Not suitable for IF.
	Hela細胞
EEA12011-08-29	clone14	WB	IF	IHC	IP
	BD Transduction		x200

	mouse	【PFA+Triton】:◎,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	MDCKII OK
	Hela細胞
ERGIC532011-08-29		WB	IF	IHC	IP
	Alexis		x1000

	mouse,IgG1	【PFA+Triton】:○,【MeOH】:◎,【TCA】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Mono	ジギトニンでは見えない。MDCKIIダメ。 Digitonin is not suitable for permeabilization. Cannot stain MDCKII
	Hela細胞
EGFR2011-08-29	1005(sc-03)	WB	IF	IHC	IP
	Santa Cruz	x200	x100-

	rabbit	【IS】:○
	Poly
	Hela細胞
EGFR2011-08-29	528(sc-120)	WB	IF	IHC	IP
	Santa Cruz		x100-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a	Immunogen: Lumenal domain
	Hela細胞
EGF2011-08-29	clone 10825	WB	IF	IHC	IP
	R&D

	mouse
	Mono, IgG1	Fof ELISA
	Hela細胞
p44/42 MAPK (ERK1/2)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech	x1000	x400
	#4695	4%PFA/PBS
	rabbit

	mouse oocyte
p44/42 MAPK (ERK1/2)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#4695	ice cold Methanol
	rabbit

	mouse oocyte
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech	x1000	x400
	#9101	4%PFA/PBS
	rabbit
		signal decreases from 3h after oocyte activation
	mouse oocyte
phopho-p44/42 MAPK (ERK1/2) (T202/Y204)2014-07-11		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#9101	ice cold Methanol
	rabbit
		signal decreases from 3h after oocyte activation
	mouse oocyte
EEA12016-11-21		WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	237002	4% PFA
	Rabbit
	IgG
	HeLa, PC12
EEA12017-08-09	C45B10	WB	IF	IHC	IP
	Cell Signaling Technology	1/1000		1/100
	3288
	Rabbit

	HeLa
EEA12011-09-27		WB	IF	IHC	IP
	BD Biosciences		1:100
	610456
	mouse
	m
	HeLa, A549, MCF7他
EEA12012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	610456	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
EEA12013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610456	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	included in Coated vesicle sampler kit
	Caco2細胞
EEA12013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610456	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	included in Coated vesicle sampler kit
	MDCK細胞

【e】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
EEA12014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610456	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
EEA12011-10-17	14/EEA1	WB	IF	IHC	IP
	BD Transduction	X1000	X100
	610457
	mouse	【3.7% formalin solution+Triton 0.2%, 5min】:○ formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	Mono,IgG1	WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	HeLa細胞, MDCK細胞
eps152013-04-11		WB	IF	IHC	IP
	BD transduction		x100
	610806	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG2a	included in Coated vesicle sampler kit
	Caco2細胞

【e】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
eps152014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610806	3%PFA (in phosphate buffer)
	mouse
	IgG2a
	mouse small intestine

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
L1 (8D9)2012-08-03		WB	IF	IHC	IP
	DSHB		x100
	8D9	3%PFA in PBS
	mouse	0.05% saponin

	PC12
ERp722012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	E72920	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
ERp722012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	E72920	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
ERp722012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	E72920	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	Caco2細胞
EEA12012-03-02		WB	IF	IHC	IP
	ABR		x100
	PA1-063	3%PFA in PBS
	rabbit	0.05% saponin
		methanol でもOK Fair staining by methanol fix.
	mouse embryonic fibroblast (MEF)
EEA12012-09-14		WB	IF	IHC	IP
	ABR		x100
	PA1-063	3%PFA in PBS
	rabbit	0.05% saponin

	Caco2
early endosomal antigen12012-01-31	EEA1	WB	IF	IHC	IP
	Affinity BioR		x100
	PA1063
	rabbit	3％PFA,　0.05%saponin
	p	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin
	PC12
early endosomal antigen12012-01-31	EEA1	WB	IF	IHC	IP
	Affinity BioR		x100
	PA1063	3%PFA (in PBS)
	rabbit
	p
	マウス小腸、膵臓
EEA12011-10-06	N-19	WB	IF	IHC	IP
	Santa Cruz		x400
	sc-6415
	Goat	4%PFA+0.1%Saponin :○
	poly	マウスのみ
	NIH3T3, マウス組織

【e】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
EEA12015-01-13		WB	IF	IHC	IP
	St. Cruz Biotechnology		x200
	SC-6415	3%PFA (in PBS)
	Goat	0.05%saponin
	IgG
	MEF

【E】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human EEA12011-08-10	N-19	WB	IF	IHC	IP
	Santa Cruz		x100
	sc6415	3%PFA (in PBS)
	goat
	p
	マウス小腸
EEA12012-09-14		WB	IF	IHC	IP
	Santa Cruz		x100
	sc6415	3%PFA in PBS
	rabbit	0.05% saponin

	Caco2

【F】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Furin2011-08-29	MON-152	WB	IF	IHC	IP
	ALEXIS		x１00

	mouse
	Mono
	Hela細胞
Furin2011-08-29	MON-148	WB	IF	IHC	IP
	ALEXIS		x１00

	mouse
	Mono
	Hela細胞
Furin2011-08-29	PA1-062	WB	IF	IHC	IP
	ABR

	rabbit
	Poly
	Hela細胞
FIP32011-08-29		WB	IF	IHC	IP
	Rockland

	rabbit
	Poly
	Hela細胞
Furin2011-08-29		WB	IF	IHC	IP
	ABR		x100

	rabbit
	Poly
	Hela細胞
Furin convertase2011-08-14		WB	IF	IHC	IP
	ALEXIS
	210-134-R100	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
mouse F4/802011-08-10		WB	IF	IHC	IP
	Serotec		x100
	MCA497R	3%PFA (in PBS)
	rat

	マウス脾臓、macrophage
Furin convertase2011-08-14		WB	IF	IHC	IP
	ABR
	PA1-062	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
furin2012-03-02		WB	IF	IHC	IP
	ABR		x100
	PA1-062	methanol
	rabbit
		3%PFA in PBSで染まらない Not stained by 3%PFA in PBS fixation
	mouse embryonic fibroblast (MEF)

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Golgin-972011-08-29	CDF4	WB	IF	IHC	IP
	Molecular Probe		x200

	mouse
	Mono, IgG1
	Hela細胞
GM1302011-08-29		WB	IF	IHC	IP
	BD Transduction		x1000

	mouse	【PFA+Triton】:◎,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	MDCKII OK.
	Hela細胞
GGA32011-08-29	clone 8	WB	IF	IHC	IP
	BD Transduction	x2500	x100

	mouse
	Mono, IgG1
	Hela細胞
Giantin2011-08-29		WB	IF	IHC	IP
	COVANCE		x1000-5000

	rabbit	【PFA+Triton】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Poly	MDCKII OK.
	Hela細胞
GGA22011-08-29	sc-19326	WB	IF	IHC	IP
	Santa Cruz

	goat
	Poly
	Hela細胞
GADD1542011-08-29		WB	IF	IHC	IP
	Santa Cruz



	Hela細胞
GGA22011-08-29	clone 27	WB	IF	IHC	IP
	BD Transduction		x100-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	MDCKII OK.
	Hela細胞
GFP2011-10-06		WB	IF	IHC	IP
	フロンティアサイエンス Frontier Science	x5000	x400
	GFP-Rb-Af2020-1
	Rabbit	4%PFA+0.1%Saponin :○ 0.1%glutalaldehyde + 4% PFA: ○
	poly

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GFP2016-10-18		WB	IF	IHC	IP
	Synaptic systems	1/10000	1/1000
	132003	4% PFA
	Rabbit
	IgG
	HeLa, PC12

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GM1302011-09-27		WB	IF	IHC	IP
	BD Biosciences		1:100
	610822
	mouse
	m
	HeLa, A549, MCF7他
GM1302011-10-04	35	WB	IF	IHC	IP
	BD Transduction		X50
	610822
	mouse	【4%PFA+0.2%Saponin】:○
	Mono, IgG1
	HL-60細胞
GM1302017-08-09		WB	IF	IHC	IP
	BD BIOSCIENCES			1/100
	610822
	Mouse

	HeLa
GM1302017-09-14	35/GM130	WB	IF	IHC	IP
	BD		1/500
	610822	4% PFA at 4oC over night
	Mouse	0.5% Triton in PBS
	IgG1
	zebrafish larvae (1 dpf/ day post fertilization)
GM1302011-09-29	35	WB	IF	IHC	IP
	BD Transduction Lab.		x200
	610823
	mouse	IF: -20℃ methanol or acetone:methanol=1:1
	mono, IgG1, κ
	HeLa細胞
GS282011-08-14		WB	IF	IHC	IP
	BD transduction		x100
	611185	3%PFA (in PBS)
	mouse
	m
	マウス小腸
GS282012-03-02		WB	IF	IHC	IP
	transduction lab		x100
	611185	3%PFA in PBS
	mouse	0.05% saponin
	IgG2a	methanol固定でも良く染まる　 Good staining by methanol fix.
	mouse embryonic fibroblast (MEF)
glucagon2015-08-07	monoclonal Ab	WB	IF	IHC	IP
	Cell signaling		400
	8223	3%PFA (in PBS)
	rabbit

	mouse pancreas
Giantin2011-10-03		WB	IF	IHC	IP
	covance		x1000
	A488-114L
	rabbit	【Formalin in PBS+0.05%Triton】:○
	Poly	Alexa Fluor 488 Labeled ホルマリンは37％ホルマリン液（ナカライ）をPBSやRingerで１/10に希釈 Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
	rat hippocampal neurons
GRASP12011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	ダメ?	ダメ
	ab30576
	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	IgG
	Hela細胞
GM1302011-11-26 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam		x300
	ab30637	4％PFA +0.1％Triton
	rabbit
	IgG
	Drosophila embryo, discs, nervous system
GP22011-10-06	2F11-C3	WB	IF	IHC	IP
	MBL		x400
	D278-3
	Rat	4%PFA+0.1%Saponin :○

	マウス腸管
GP22012-11-13	2F11-C3	WB	IF	IHC	IP
	MBL		2.5ug/ml
	D278-3	3%PFA (in PB)
	Rat
	IgG2 κ	acinar細胞のzymogen顆粒が非常に強く染まる。 Zymogen granules are very strongly stained in IF
	マウス膵臓
Golgi 58k Protein (ERGIC53)2019-09-10	58K-9	WB	IF	IHC	IP
	SIGMA		x50
	G2404	3.7% Formaldehyde (in PBS)
	mouse	Methanol (-20˚C)
	IgG1
	NRK
GM1302012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	G65120	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
GM1302012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G65120	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
GM1302012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G65120	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK
GM1302019-09-10	35	WB	IF	IHC	IP
	Transduction Laboratories		x100
	G65120	3.7% Formaldehyde (in PBS)
	mouse	Methanol (-20˚C)
	IgG1
	HEL
GS152012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	G72420	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GS152014-02-04		WB	IF	IHC	IP
	Transduction		x100
	G72420	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GS272012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	G76220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GS272014-02-04		WB	IF	IHC	IP
	Transduction		x100
	G76220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
GS282012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G83820	3%PFA in PBS
	mouse	0.05% saponin
	IgG2a	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
GS282012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G83820	3%PFA in PBS
	mouse	0.05% saponin
	IgG2a	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK
Golgin842012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	G93720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
golgin842012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G93720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
golgin842012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G93720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK
GCC88（GCC1)2012-10-03		WB	IF	IHC	IP
	Genetex		x200
	GTX120148	3%PFA (in PBS)
	rabbit	0.1% Triton
		Golgiが染まります Stains the Golgi
	Hela細胞
GCC185（GCC2)2012-10-03		WB	IF	IHC	IP
	Sigma		x200
	HPA035849	3%PFA (in PBS)
	rabbit	0.1% Triton
		Golgiが染まります Stains the Golgi
	Hela細胞
rat GRP782011-08-10	bip	WB	IF	IHC	IP
	Affinity BioR		x100
	PA1014	3%PFA (in PBS)
	rabbit

	マウスMEF
rat GRP782011-08-10	bip	WB	IF	IHC	IP
	Affinity BioR
	PA1014
	rabbit


GM1302011-10-04		WB	IF	IHC	IP
	MBL		X1000
	PM061
	rabbit	【4%PFA+0.2%Saponin】:○
	Poly
	HL-60細胞
GlcCer2011-08-10		WB	IF	IHC	IP
	Glycobiotech		x100
	RAS0010	3%PFA (in PBS), methanol
	rabbit

	マウス小腸

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
γ-tubulin2014-06-04	H-183	WB	IF	IHC	IP
	SANTA CRUZ		x200		x400
	sc-10732	MeOH
	rabbit
	IgG
	HeLa・mouse oocyte （IF only）

【G】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human GABARAP2011-08-10	Y-13	WB	IF	IHC	IP
	Santa Cruz		x100
	sc26515	3%PFA (in PBS)
	goat
	p
	マウス小腸

【g】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
γ-tubulin2014-06-04	DQ-19	WB	IF	IHC	IP
	SIGMA	x1000			x400
	T3195
	rabibt
	IgG
	HeLa

【H】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Hrs-22011-08-29	A-5	WB	IF	IHC	IP
	ALEXIS		x50

	mouse	【PFA+Triton】:×,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG2a	MeOH固定 Fixation by MeOH is best.
	Hela細胞
bovine MPR (22d4)2012-08-03		WB	IF	IHC	IP
	DSHB		x100
	22d4	3%PFA in PBS
	mouse	0.05% saponin

	PC12
bovine MPR (86f7)2012-08-03		WB	IF	IHC	IP
	DSHB		x100
	86f7	3%PFA in PBS
	mouse	0.05% saponin

	PC12
human SNAP-252012-08-03		WB	IF	IHC	IP
	Chemicon		x100
	AB5871P	3%PFA in PBS
	goat	0.05% saponin

	PC12
mouse LAMP2 (ABL93)2012-08-03		WB	IF	IHC	IP
	DSHB		x100
	ABL93	3%PFA in PBS
	rat	0.05% saponin

	PC12
Hsp902012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	H38220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Hsp902012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H38220	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
Hsp902012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H38220	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
Hsp902012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H38220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	calnexinより染色弱い Weaker staining than calnexin Ab
	Caco2細胞
Hsp902012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H38220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Hsp1102012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H44620	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
Hsp1102012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H44620	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
Hsp1102012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H44620	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	Caco2細胞
Hsp702012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	H53220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Hsp702012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H53220	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
Hsp702012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H53220	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
Hsp702012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H53220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	染色弱い Weak staining
	Caco2細胞
Hsp702012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H53220	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
HSF42012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H65520	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
HSF42012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	H65520	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
HSF42012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	H65520	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	Caco2細胞

【K】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
KDEL-R2011-08-29	VAA-PT048	WB	IF	IHC	IP
	StressGen		x200

	mouse
	Mono, IgG1
	Hela細胞
KDEL2011-11-26	SPA-827	WB	IF	IHC	IP
	stressgen		x50
	ADI-SPA-827D	4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
KDEL receptor2011-11-26	VAA-PT048	WB	IF	IHC	IP
	stressgen		x50
	ADI-VAA-PT048D	4％PFA +0.1％Triton
	mouse

	Drosophila embryo, discs, nervous system
KDEL2011-08-12		WB	IF	IHC	IP
	Stressgen
	SPA827
	mouse
	m	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

KDEL2011-08-10		WB	IF	IHC	IP
	Stressgen		x100
	SPA827	3%PFA (in PBS)
	mouse
	m
	マウス膵、精巣
KDEL2012-08-03		WB	IF	IHC	IP
	Stressgen		x100
	SPA827	3%PFA in PBS
	mouse	0.05% saponin

	PC12
KDEL2013-01-19	10C3	WB	IF	IHC	IP
	Stressgen		x100
	SPA827	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG2a	弱いが、1/5くらいの細胞でそまる Very weakly stained in -20% of all cells
	Caco2細胞
KDEL receptor2011-08-14		WB	IF	IHC	IP
	Stressgen		x100
	VAA-PT048	3%PFA (in PBS)
	mouse
	m
	マウス小腸

【L】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Lamp2 (CD107b)2011-08-29	H4B4	WB	IF	IHC	IP
	BD Pharmingen		x1000

	mouse	【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
Lamp1 (CD107a)2011-08-29	H4B3	WB	IF	IHC	IP
	BD Pharmingen		x5000-10000

	mouse	【PFA+Triton】:○,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1k	MDCKII ダメ、saponin使うとかなり明るい。 Not suitable for MDCKII. Good staining by saponin permeabilization.
	Hela細胞
LBPA2011-08-29	6C4	WB	IF	IHC	IP
	Echelon		x100

	mouse	【PFA+Triton】:×,【PFA+Saponin】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1k
	Hela細胞
LDLR2011-08-29	C7	WB	IF	IHC	IP
	ATCC	ダメ（？）	x100

	mouse	【PFA+Triton】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	mono	Hybridoma Supernatant
	Hela細胞
Lamp12011-10-06	1D4B	WB	IF	IHC	IP
	eBioscience		x200
	14-1071-81
	Rat	4%PFA+0.1%Saponin :○
	mono, IgG2a	マウスのみ
	NIH3T3, マウス組織
Lamp12011-10-06	H4A3	WB	IF	IHC	IP
	eBioscience		x400
	14-1079-80
	mouse	4%PFA+0.1%Saponin :○
	IgG1	ヒトのみ
	HeLa
mouse LAMP12011-08-10	1D4B	WB	IF	IHC	IP
	DSHB		x100
	1D4B	3%PFA (in PBS)
	rat
		ImEMにも使用可能 Applicable for ImEM
	マウス小腸
LAMP12011-09-27	1D4B	WB	IF	IHC	IP
	BD Biosciences		1:100
	553792
	rat
	m	mouse細胞専用
	mouse (MEF, NIH3T3)
bovine LDLR2011-08-10		WB	IF	IHC	IP
	Progen		x100
	61087	3%PFA (in PBS), methanol
	mouse
	m
	マウス小腸
mouse LAMP22011-08-12		WB	IF	IHC	IP
	DSHB
	ABL93
	rat
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

mouse LAMP22011-08-10	ABL93	WB	IF	IHC	IP
	DSHB		x100
	ABL93	3%PFA (in PBS)
	rat
		ImEMには使えない（GAで失活）が、PFAでは一番よい Not suitable for ImEM because antigenicity is lost by GA. Stains LAMP2 best of all lamp2 Abs by PFA fix.
	マウス小腸
mouse LDLR2011-08-10	NS0	WB	IF	IHC	IP
	R&D Systems	x1000	x100
	AF2255	3%PFA (in PBS)
	goat

	マウス小腸、腎臓
mouse LAMP22011-08-10	GL2A7	WB	IF	IHC	IP
	DSHB
	GL2A7
	rat


human LAMP12011-08-10	H4A3	WB	IF	IHC	IP
	DSHB		x100
	H4A3	3%PFA (in PBS)
	mouse

	Caco2細胞
lamp12012-09-14	H4A3	WB	IF	IHC	IP
	DSHB		x100
	H4A3	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	もっと薄められる Can be diluted more
	Caco2
lamp12012-09-14	H4A3	WB	IF	IHC	IP
	DSHB		x100
	H4A3	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	H4A3, 1D4Bとも染まらず Not stained by H4A3 or 1D4B Abs
	MDCK
human LAMP22011-08-10	H4B4	WB	IF	IHC	IP
	DSHB		x100
	H4B4	3%PFA (in PBS)
	mouse

	Caco2細胞
lamp22012-09-14	H4B4	WB	IF	IHC	IP
	DSHB		x100
	H4B4	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	もっと薄められる Can be diluted more
	Caco2
lamp22012-09-14	H4B4	WB	IF	IHC	IP
	DSHB		x100
	H4B4	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	ABL93, H4B4, GL2A7全てダメ Not stained by ABL93, H4A4 or GL2A7 Abs
	MDCK
LC3B2011-08-10		WB	IF	IHC	IP
	sigma
	L7543
	rabbit
	p

LC32011-09-27		WB	IF	IHC	IP
	MBL	1:1000	1:500~1:1000
	PM036
	rabbit
	p
	MEF ,NIH3T3, HeLa, A549, MCF7他
LAMP12011-09-27	H4A3	WB	IF	IHC	IP
	Santa Cruz		1:500~1:1000
	sc-20011
	mouse
	m	Human細胞専用
	human (HeLa, A549, MCF7)
mouse LDLR2011-08-10	M-20	WB	IF	IHC	IP
	Santa Cruz		x100
	sc11826	3%PFA (in PBS), methanol
	goat
	p
	マウス小腸
LBPA(BMP)2016-12-14	6C4	WB	IF	IHC	IP
	Echelon		1/500
	Z-PLBPA	4% PFA
	Mouse
	IgG
	HeLa, PC12

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
CI-M6PR (IGFIIR)2011-08-29		WB	IF	IHC	IP
	BD Transduction	x250

	mouse
	Mono	Rat specific, Immunogen: extracellular domain
	Hela細胞
CI-M6PR2011-08-29	2G11	WB	IF	IHC	IP
	ABR		x10-20

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞
Man II2011-08-29	53FC3	WB	IF	IHC	IP
	BAbCO		x1000x500

	mouse
	Mono, IgG2b	Ratしか使えません。 Only applicable for rat cells and tissues.
	Hela細胞
bovine MPR2011-08-12	22d4	WB	IF	IHC	IP
	DSHB
	22d4
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

bovine MPR2011-08-10	22d4	WB	IF	IHC	IP
	DSHB		x100
	22d4	3%PFA (in PBS)
	mouse
		マウス小腸はダメ Not suitable for mouse small intestine.
	Caco2, MDCK
bovine MPR2011-08-12	86f7	WB	IF	IHC	IP
	DSHB
	86f7
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

bovine MPR2011-08-10	86f7	WB	IF	IHC	IP
	DSHB		x100
	86f7	3%PFA (in PBS), methanol
	mouse

	マウス小腸
human M6PR2011-08-10		WB	IF	IHC	IP
	GenWay Biotech		x100
	A21444	3%PFA (in PBS), methanol
	chicken(IgY)
	p
	マウス小腸
CI-M6PR2011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab32815
	rabbit
	IgG
	Hela細胞

【m】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	4%PFA/PBS
	mouse
	IgG1
	mouse oocyte (pronucleus)
mAb4142014-08-01		WB	IF	IHC	IP
	COVANCE		x400
	MMS-120P	ice cold Methanol
	mouse
	IgG1
	mouse oocyte (pronucleus)

【M】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human MAP LC32011-08-10	F-14	WB	IF	IHC	IP
	Santa Cruz		x100
	sc16756	3%PFA (in PBS), methanol
	goat
	p
	マウス小腸
human MAP LC32011-08-10	H-50	WB	IF	IHC	IP
	Santa Cruz		x100
	sc28266	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
NGF-receptor　2011-08-12	p75	WB	IF	IHC	IP
	NeoMarkers

	mouse
	m	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

NSF2011-08-29		WB	IF	IHC	IP
	Trunsduction	x200

	mouse
	mono
	Hela細胞
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup982014-08-01		WB	IF	IHC	IP
	Cell Signaling Tech		x400
	#2598	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
Nup1332014-07-11		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup1332014-08-01		WB	IF	IHC	IP
	Proteintech Group		x400
	12405-1-AP	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	4%PFA/PBS
	mouse
	IgG2b
	mouse oocyte (pronucleus)
Nup622014-08-01		WB	IF	IHC	IP
	BD transduction		x400
	610497	ice cold Methanol
	mouse
	IgG2b
	mouse oocyte (pronucleus)

【n】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
beta NAP2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610892	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

【N】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	4%PFA/PBS
	rabbit

	mouse oocyte (pronucleus)
Nup352014-08-01		WB	IF	IHC	IP
	SIGMA		x400
	HPA018401	ice cold Methanol
	rabbit

	mouse oocyte (pronucleus)

【O】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
OCRL12011-08-29		WB	IF	IHC	IP
	LIFESPAN		x100-

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:△,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
OCRL12011-08-29		WB	IF	IHC	IP
	Santa Cruz	ダメ?	ダメ

	goat	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
OCRL12011-08-29	4A6	WB	IF	IHC	IP
	Abnova	x500-	ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PDI2011-08-29	1D3	WB	IF	IHC	IP
	StressGen

	mouse	【PFA+Triton】:○,【PFA+Saponin】:×,【MeOH】:○,【TCA】:△【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
p2302011-08-29		WB	IF	IHC	IP
	BD transduction	x250	x200

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	MDCKII OK
	Hela細胞
p24（HIV-1 a-CA)2011-08-29	13-102-100	WB	IF	IHC	IP
	ABI

	mouse
	Mono, IgG1
	Hela細胞
p17（HIV-1 a-MA)2011-08-29	13-103-1-100	WB	IF	IHC	IP
	ABI

	mouse
	Mono, IgG1
	Hela細胞
p47A2011-08-29	clone 18	WB	IF	IHC	IP
	BD Transduction	x250

	mouse
	Mono, IgG1
	Hela細胞
PACS12011-08-29	60B233	WB	IF	IHC	IP
	Antagene		ダメ?

	rabbit	【PFA+Saponin】:×【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS
	poly
	Hela細胞
PACS-1a2011-08-29	clone 4	WB	IF	IHC	IP
	BD Transduction	x200	ダメ?

	mouse	【PFA+Saponin】:×【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
PI(4,5)P22011-08-29		WB	IF	IHC	IP
	Echelon		x100

	mouse
	Mono, IgM	PFA+Saponin(+biotin+streptavidin)でも微妙 Very weak staining by PFA+Saponin(+biotin+streptavidin)
	Hela細胞
PI(4)P2011-08-29		WB	IF	IHC	IP
	Echelon		x500-

	mouse	【PFA+Triton】:×,【PFA+Saponin】:○,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgM
	Hela細胞
PI 4-kinase?α2011-08-29		WB	IF	IHC	IP
	ABGENT	x100-	ダメ

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
PI 4-kinase?β2011-08-29		WB	IF	IHC	IP
	ABGENT	x100-	ダメ

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
PI 4-kinase?α2011-08-29		WB	IF	IHC	IP
	ABGENT	ダメ?	ダメ

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
PI 4-kinase?β2011-08-29	clone 7	WB	IF	IHC	IP
	BD Pharmingen	x500	x5000-10000

	mouse	【PFA+Triton】:◎,【PFA+Saponin】:◎,【IS】:○,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞
PI 4-kinase?β2011-08-29		WB	IF	IHC	IP
	Upstate	x500-	x400-

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:◎,【IS】:△,【MeOH】:△【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	Saponinがbetter。 Can get better staining by saponin permeabilization.
	Hela細胞
NGF-receptor (p75)2012-08-03		WB	IF	IHC	IP
	NeoMarkers		x100
		3%PFA in PBS
	mouse	0.05% saponin

	PC12

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PKD 12014-01-07		WB	IF	IHC	IP
	Cell Signaling Technology	x500	x100
	2052	3%PFA (in PBS)
	rabbit
	IgG	WB: cross reaction with PKD2
	WB: several tissues; IF: Lung

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Porin2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (MitoSciences)
	459500
	Mouse
	monoclonal

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PKD 32014-01-07		WB	IF	IHC	IP
	Cell Signaling Technology	x1000
	5655
	rabbit
	IgG
	WB: several tissues

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p47A2013-07-04		WB	IF	IHC	IP
	BD transduction		x100
	610900	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	fine punctate staining like adaptin alpha , included in Coated vesicle sampler kit
	Caco2細胞

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p47A2014-04-03		WB	IF	IHC	IP
	BD Transduction		x100
	610900	3%PFA (in phosphate buffer)
	mouse
	IgG1
	mouse small intestine

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p2302011-09-27		WB	IF	IHC	IP
	BD Biosciences		1:100
	611280
	mouse
	m	trans Golgi
	HeLa, A549, MCF7他
p150glued2011-10-06	12/P150GLUED	WB	IF	IHC	IP
	BD bioscience		x400
	612709
	mouse	4%PFA+0.1%Saponin :○
	IgG1
	HeLa
Pep122011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (Molecular Probes)
	A-21273
	Mouse
	monoclonal

PIP32011-10-06	RC6F8	WB	IF	IHC	IP
	Invitrogen		x400
	A-21328
	mouse	1% Glutalaldehyde + 4%PFA + 0.1%Saponin
	IgM	Glutaladehyde固定は必須。固定後すぐに次のステップへ。
	HeLa
Phosphoglycerate Kinase2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (MitoSciences)
	A-6457
	Mouse
	monoclonal

human PKC mu2011-08-10 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab5284
	rabbit
	IgG

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
podocalyxin2014-02-04		WB	IF	IHC	IP
	R&D systems		x100
	AF1658	3%PFA (in PBS)
	goat	0.05%saponin

	MDCK

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human p75 pAb2011-08-12		WB	IF	IHC	IP
	Promega
	G3231
	rabbit
	ｐ	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

human p75 pAb2012-08-03		WB	IF	IHC	IP
	Promega		x100
	G3231	3%PFA in PBS
	rabbit	0.05% saponin

	PC12
p230tranGolgi2012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	G88620	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
PIP22011-10-06	2C11	WB	IF	IHC	IP
	LifeSpanBIoscience		x400
	LS-C49060-100
	mouse	1% Glutalaldehyde + 4%PFA + 0.1%Saponin
	IgM	Glutaladehyde固定は必須。固定後すぐに次のステップへ。
	HeLa

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PKD 12014-01-07		WB	IF	IHC	IP
	Sigma	x100000	x100
	P 3987	3%PFA (in PBS)
	rabbit
	IgG	WB: cross reaction with PKD2
	WB: several tissues; IF: Lung

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
p1152012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	P67420	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
PDI2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	P71720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
PDI2012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	P71720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
PDI2012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	P71720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
PDI2012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	P71720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	Caco2細胞
PDI2012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	P71720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
p622011-08-14		WB	IF	IHC	IP
	MBL
	PM045
	rabbit


p62/SQSTM2011-09-27		WB	IF	IHC	IP
	MBL	1:2000	1:500
	PM045
	rabbit
	p
	MEF, HeLa, A549, MCF7他

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PKD 12014-01-07		WB	IF	IHC	IP
	St. Cruz Biotechnology C-20	x100000	x100
	SC-639	3%PFA (in PBS)
	rabbit
	IgG	WB: cross reaction with PKD2
	WB: several tissues; IF: Lung
PKD 12014-01-07		WB	IF	IHC	IP
	St. Cruz Biotechnology D-20	x2000	x100
	SC-935	3%PFA (in PBS)
	rabbit
	IgG	WB: cross reaction with PKD2
	WB: several tissues; IF: Lung

【P】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human PKC m2011-08-10	D-20	WB	IF	IHC	IP
	Santa Cruz
	sc935
	rabbit
	p

PDI2012-03-02		WB	IF	IHC	IP
	Stressgen		x100
	SPA-891	methanol
	rabbit
		3%PFA in PBSでも染まるが弱い Only weakly stained by 3%PFA in PBS fixation
	mouse embryonic fibroblast (MEF)
rat PDI2011-08-10		WB	IF	IHC	IP
	Stressgen
	SPA891	3%PFA (in PBS)
	mouse
	m	IFＯＫ？
	マウスＭＥＦ
PDI2013-01-19	1D3	WB	IF	IHC	IP
	Stressgen		x100
	SPA891	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1	そまる細胞の割合は他のER markerより高い Higher rate of cells are stained by this Ab than other ER Abs.
	Caco2細胞

【p】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
PDI2014-02-04		WB	IF	IHC	IP
	StressGen		x100
	SPA891	3%PFA (in PBS)
	mouse	0.05%saponin

	MDCK

【R】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Rab72011-08-14		WB	IF	IHC	IP
	MBL

	rabbit
	p

RUFY12011-08-29	3A5	WB	IF	IHC	IP
	Abnova		ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a
	Hela細胞
Rabaptin52011-08-29	C-20	WB	IF	IHC	IP
	Santa Cruz	x500-1000	x50

	goat
	Poly
	Hela細胞
Rabaptin52011-08-29	N-19	WB	IF	IHC	IP
	Santa Cruz

	goat
	Poly
	Hela細胞
Rabaptin-52011-08-29	clone 20	WB	IF	IHC	IP
	BD Transduction		ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
Rab112011-08-29		WB	IF	IHC	IP
	Zymed	x500	x100-

	rabbit	【PFA+Triton】:△,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly	WB：Rab11aのみ認識、IFで見えるendosomeのシグナルはKDで半分ぐらいしか消えない。 Only recognize Rab11a by WB. Only half of the endosome staining dissapears in Rab11a KD cells.
	Hela細胞
Rab112011-08-29	clone 47	WB	IF	IHC	IP
	BD Transduction	x200-	x200

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×,【TCA】:○、◎(+CanGetSignal (from TOYOBO Inc.))、BlockingはO/N【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a	WB: Rab11a, b両方認識する Recognize both Rab11a and Rab11b by WB.
	Hela細胞
Rab 5B2011-08-29	A-20	WB	IF	IHC	IP
	Santa Cruz

	rabbit
	Poly
	Hela細胞
Rab 5A2011-08-29	sc-309	WB	IF	IHC	IP
	Santa Cruz		x100

	rabbit
	Poly
	Hela細胞
Rab52011-08-29	clone 1	WB	IF	IHC	IP
	BD Transduction	x200-	x100-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2a	PFAでもいいが、saponinがbetter Saponin permeabilization is better.
	Hela細胞
Rab 42011-08-29	D-20	WB	IF	IHC	IP
	Santa Cruz

	rabbit
	Poly
	Hela細胞
Rab42011-08-29	clone 7	WB	IF	IHC	IP
	BD Transduction	x100-	ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	endosome likeのsignalが見えるが、siRab4で消えない。 Recognize endosome-like signals which cannot disappear by siRab4.
	Hela細胞
Rac12011-08-29		WB	IF	IHC	IP
	Chemicon

	mouse
	Mono
	Hela細胞
Rac12011-08-29	clone 102	WB	IF	IHC	IP
	BD Pharmingen		ダメ

	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2b
	Hela細胞

【r】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
rab52016-08-10	621.3	WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	108011	4% PFA
	Mouse
	IgG
	HeLa, PC12

【R】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Rab352011-08-31		WB	IF	IHC	IP
	ProteinTech Group
	11329-2-AP
	rabbit
	p

Rab272011-08-31		WB	IF	IHC	IP
	IBL
	18975	4%PFA (in PBS)
	rabbit
	p	Rab27A,B共に認識 recognize both Rab27a and Rab27b
	melan-a
Rab72011-08-31		WB	IF	IHC	IP
	Cell signaling
	2094S	4%PFA (in PBS)
	rabbit
	p
	melan-a
Rab82012-04-17	4/Rab4	WB	IF	IHC	IP
	Transduction	x5,000~
	61-844
	mouse
	IgG2b

Rab32011-08-31		WB	IF	IHC	IP
	BD transduction
	610379	4%PFA (in PBS)
	mouse
	m
	マウス海馬ニューロン, PC12
Rab272011-08-14		WB	IF	IHC	IP
	BD transduction
	610596
	mouse
	m

Rab112011-08-14		WB	IF	IHC	IP
	BD transduction
	610656
	mouse
	m

Rab112011-08-31		WB	IF	IHC	IP
	BD transduction
	610656	4%PFA (in PBS)
	mouse
	m	Rab11A,B共に認識 recognize both Rab11a and Rab11b
	MEF
Rab112011-09-27	clone 47/Rab11	WB	IF	IHC	IP
	BD		100
	610656
	mouse
	m	TCA (Can Get Signal Immunostain 使用） http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld96/technical/canget96tr01.pdf http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld95/technical/canget95tr02.pdf
	HeLa
Rab82011-08-14		WB	IF	IHC	IP
	BD transduction	x1000	x100
	610845	3%PFA (in PBS), methanol
	mouse
	m	Rab8a,b共に認識 Recognize both Rab8a and Rab8b
	マウス小腸
Rab112011-08-14		WB	IF	IHC	IP
	Zymed
	71-5300
	rabbit
	p

Rab112011-08-31		WB	IF	IHC	IP
	Invitrogen
	71-5300	4%PFA (in PBS)
	rabbit
	p	Rab11A,B共に認識 Recognize both Rab11a and Rab11b
	マウス海馬ニューロン, MEF
Rab72016-12-14		WB	IF	IHC	IP
	Cell signaling	1/1000	1/500
	9367	4% PFA
	Rabbit
	IgG
	HeLa, PC12
Rabenosyn-52011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	ダメ?	ダメ
	ab21196
	goat	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	IgG
	Hela細胞

【r】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
rab52014-10-06		WB	IF	IHC	IP
	Abcam		x500
	ab31261	4%PFA (in phosphate buffer)
	rabbit

	Drosohila imaginal disc

【R】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Rab142011-08-29 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam	x500	x100
	ab40938
	rabbit
	IgG	Saponinがbetter 汚い Saponin permeabilization is better.
	Hela細胞
Rab33B2011-08-31		WB	IF	IHC	IP
	Frontier Science
	D5	4%PFA (in PBS)
	mouse
	m	MEF, NIH3T3ではIF不可。Rab33Aは認識せず。 Not suitable for IF in MEFs and 3T3. Not recognize Rab33A.
	HeLa, HeLaS3
Rab1A2011-08-31		WB	IF	IHC	IP
	Abnova
	H00005861-M07A
	mouse
	m

Rab27A2011-08-31		WB	IF	IHC	IP
	Abnova
	H00005873-M02
	mouse
	m

Rab42011-08-14		WB	IF	IHC	IP
	Stressgen
	KAP-GP005
	rabbit
	p

rab42011-09-22		WB	IF	IHC	IP
	Stressgen		1/500
	KAP-GP005
	rabbit	4 % PFA fix, PBS+0.05 %Tween20+ 0.5 % TSA block+1 % Donkey serum
	serum
	mouse E6.5 embryo.MEF
Rubicon2011-09-27	1H6	WB	IF	IHC	IP
	MBL	1:1000
	M170-3
	mouse
	m	WBでhumanのみ検出可能

Rubicon2011-09-27		WB	IF	IHC	IP
	MBL	1:1000
	PD027
	rabbit
	p	WBでhumanのみ検出可能

Rab52011-08-14		WB	IF	IHC	IP
	BD transduction
	R28720
	mouse
	m

Rer12014-06-19		WB	IF	IHC	IP
	Sigma-Aldrich	x1000	x200
	R4407	3%PFA (in PBS)
	Rabbit	0.05% Saponin
	IgG1	highly specific for human by WB and IF
	HeLa
Rab72011-08-29		WB	IF	IHC	IP
	Sigma	ダメ
	R4779
	rabbit	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
Rab82011-08-29		WB	IF	IHC	IP
	Sigma		x300-500
	R5530
	rabbit	【PFA+Triton】:○,【TCA】:◎【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS
	Poly, anti-sera,IgGfraction
	Hela細胞
Rab82012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	R66320	3%PFA in PBS
	mouse	0.05% saponin
	IgG2b	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
Rab82012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	R66320	3%PFA in PBS
	mouse	0.05% saponin
	IgG2b	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK
Rab72011-08-29	Rab7-117	WB	IF	IHC	IP
	Sigma	ダメ
	R8779
	mouse	【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG2b
	Hela細胞
Rab82012-04-17		WB	IF	IHC	IP
	SantaCrus	x500-1,000
	sc-26578
	goat


Rab5 (S-19)2011-10-17		WB	IF	IHC	IP
	Santa Cruz	X500
	sc-309
	rabbit
	Poly	WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
	HeLa細胞
Rab6A2012-04-17	38-TB	WB	IF	IHC	IP
	SantaCruz	x1,000
	sc-81913
	mouse


human Rab72011-08-10	H-50	WB	IF	IHC	IP
	Santa Cruz		x100
	sc10767	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
human Rab62011-08-12	C-19	WB	IF	IHC	IP
	Santa Cruz
	sc310
	rabbit
	p	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

human Rab62011-08-10	C-19	WB	IF	IHC	IP
	Santa Cruz		x100
	sc310	3%PFA (in PBS)
	rabbit
	p
	マウスＭＥＦ、ＷＢ
human Rab6 (C-19)2012-08-03		WB	IF	IHC	IP
	Santa Cruz		x100
	sc310	3%PFA in PBS
	rabbit	0.05% saponin

	PC12
human Rab9A2011-08-10	C-18	WB	IF	IHC	IP
	Santa Cruz		x100
	sc6562	3%PFA (in PBS), methanol
	goat
	p
	マウス小腸
human Rab112011-08-10	C-19	WB	IF	IHC	IP
	Santa Cruz		x100
	sc6565	3%PFA (in PBS), methanol
	goat
	p
	マウス小腸

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
SREBP22011-08-29	ID2	WB	IF	IHC	IP
	MBL	x500	ダメ

	mouse,IgG1
	Mono
	Hela細胞
SREBP22011-08-29		WB	IF	IHC	IP
	Cayman	ダメ	ダメ

	rabbit
	Poly
	Hela細胞
S2P2011-08-29		WB	IF	IHC	IP
	Cell Signaling

	rabbit
	Poly
	Hela細胞
S2P2011-08-29		WB	IF	IHC	IP
	Santa Cruz

	rabbit
	Poly
	Hela細胞
SNX12011-08-29	Clone 51	WB	IF	IHC	IP
	BD Transduction		x100-

	mouse	【PFA+Triton】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1
	Hela細胞
Syntaxin 52011-08-29		WB	IF	IHC	IP
	SYSY		x200-

	rabbit
	Poly	もう少し希釈倍率を上げても良いと思います(IF) Can be diluted further for IF
	Hela細胞
Syntaxin 62011-08-29	610635	WB	IF	IHC	IP
	BD Transduction	x100

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
Syntaxin 62011-08-29		WB	IF	IHC	IP
	SySy	x200

	rabbit
	Poly	BDのものよりWB良い。 Better Ab for WB than BD Ab
	Hela細胞
Syntaxin 82011-08-29		WB	IF	IHC	IP
	SySy		x200

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
Syntaxin12/132011-08-29		WB	IF	IHC	IP
	SySy

	rabbit	【PFA+Triton】:×【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Poly
	Hela細胞
Syntaxin 162011-08-29		WB	IF	IHC	IP
	SySy		x1000-

	rabbit	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
sec152011-08-29		WB	IF	IHC	IP
	Santa Cruz

	rabbit
	Poly
	Hela細胞
sec15B2011-08-29		WB	IF	IHC	IP
	Santa Cruz	x200

	goat
	Poly	IFは試してない,WBも微妙 Not tested for IF. Not good staining for WB.
	Hela細胞
sec31A2011-08-29		WB	IF	IHC	IP
	BD Transduction		x200?

	mouse,IgG1	【PFA+Triton】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono
	Hela細胞
STｘB2011-08-29	13C4	WB	IF	IHC	IP
	ATCC		x100

	mouse
	Mono
	Hela細胞
syntaxin2011-11-26	8C3	WB	IF	IHC	IP
	Developmental study hybridoma bank
		4％PFA +0.1％Triton
	mouse
	IgG
	Drosophila embryo, discs, nervous system
syntaxin32013-07-04		WB	IF	IHC	IP
	synaptic systems	x1000

	rabbit
		no staining
	mouse brain, intestine

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
synaptophysin 12016-10-18	7.2	WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	101011	4% PFA
	Mouse
	IgG
	primary neuron, PC12

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
synaptobrevin/VAMP 22011-08-12		WB	IF	IHC	IP
	Synaptic Sy
	104211
	mouse
	m	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

synaptobrevin/VAMP 22011-08-10		WB	IF	IHC	IP
	Synaptic Sy	x1000
	104211	3%PFA (in PBS)
	mouse
	m
	マウス

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
synaptobrevin 2 (VAMP2)2016-08-10	69.1	WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	104211	4% PFA
	Mouse
	IgG
	primary neuron, PC12
synaptotagmin 12016-10-18	41.1	WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	105011	4% PFA
	Mouse
	IgG
	primary neuron, PC12
syntaxin 12016-09-08	78.2	WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	110011	4% PFA
	Mouse
	IgG
	primary neuron, PC12

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
syntaxin 32011-08-10		WB	IF	IHC	IP
	Synaptic Sy		x100
	110033	3%PFA (in PBS), methanol
	rabbit
	p
	WB, マウス小腸共

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
syntaxin 62016-09-08		WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	110062	4% PFA
	Rabbit
	IgG
	HeLa, PC12
syntaxin 12/132016-08-10		WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	110133	4% PFA
	Rabbit
	IgG
	HeLa, PC12

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
SNAP-232011-08-10		WB	IF	IHC	IP
	Synaptic Sy		x100
	111202	3%PFA (in PBS)
	rabbit
	p	自家製より弱いのとspecificity低い Weaker and lower specificity than homemade antibody
	マウス脳
Syntaxin42011-08-14		WB	IF	IHC	IP
	BD transduction
	610439
	mouse
	m

Syntaxin42012-11-13	49	WB	IF	IHC	IP
	BD Transduction	x1000
	610439
	mouse
	IgG1
	マウス膵臓ライセート
Sec82011-08-14		WB	IF	IHC	IP
	BD transduction
	610658
	mouse
	m

SYBL12011-08-10 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam		x100
	ab36195
	mouse
	IgG1	WBで染まるが、ＫＯでもバンドが出るため、VAMP7以外のものを認識しているようだ Can detect proteins by WB even in VAMP7 knockout mice, so some other proteins may be detected.

SNAP232011-08-10 About Product	Polyclonal	WB	IF	IHC	IP
	Abcam
	ab50357
	rabbit
	IgG
	？
syntaxin 32011-08-10		WB	IF	IHC	IP
	Chemicon		x100
	AB5448	3%PFA (in PBS), methanol
	rabbit
	p
	WB, マウス小腸共
syntaxin32013-07-04		WB	IF	IHC	IP
	chemicon	x1000
	AB5448
	rabbit
		nonspecific band at ca.30k even in KO tissue
	mouse brain, intestine
human SNAP-252011-08-12		WB	IF	IHC	IP
	Chemicon
	AB5871P
	goat
	p	PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）染まらなかった Unsuccessful staining for IF of PC12 with 3%PFA, 0.05%saponin

human SNAP-252011-08-10		WB	IF	IHC	IP
	Chemicon
	AB5871P
	goat
	p
	？
syntaxin 32011-08-10		WB	IF	IHC	IP
	GeneTex		x100
	GTX24113	3%PFA (in PBS), methanol
	rabbit
	p
	WB, マウス小腸共
syntaxin32013-07-04		WB	IF	IHC	IP
	gene tex	x1000
	GTX24113
	rabbit
		nonspecific band at ca.30k even in KO tissue
	mouse brain, intestine

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
syntaxin32013-12-04		WB	IF	IHC	IP
	gene tex		x100
	GTX24113	3%PFA (in PBS)
	rabbit	0.05%saponin

	MDCK

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
Syntaxin32011-08-14		WB	IF	IHC	IP
	Atlas
	HPA002191	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
syntaxin32013-07-04		WB	IF	IHC	IP
	atlas antibodies (sigma)	x1000
	HPA002191
	rabbit
		no staining
	mouse brain, intestine
S2P2011-08-29		WB	IF	IHC	IP
	SIGMA
	HPA005494
	rabbit
	Poly
	Hela細胞
Syntaxin12011-08-14	HPC-1	WB	IF	IHC	IP
	MBL
	K0117-3
	mouse
	m

human synaptobrevin2011-08-10	VAMP	WB	IF	IHC	IP
	Chemicon
	MAB335
	mouse
	m

syntaxin12015-10-06	HPC-1	WB	IF	IHC	IP
	Sigma	5000
	S0664
	mouse

	pancreas lysate
syntaxin12015-10-06	HPC-1	WB	IF	IHC	IP
	Sigma		100
	S0664	3%PFA (in PBS)
	mouse

	mouse primary neuron, neural progenitor cell, pancreas
Syntaxin62012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	S55420	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
syntaxin62012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	S55420	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
syntaxin62012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	S55420	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK
Syntaxin 32011-08-10		WB	IF	IHC	IP
	Sigma		x100
	S5547	3%PFA (in PBS), methanol
	rabbit

	WB, マウス小腸共
syntaxin32013-07-04		WB	IF	IHC	IP
	sigma	x1000
	s5567
	rabbit
		nonspecific band at ca.30k even in KO tissue
	mouse brain, intestine
STI1/Hop-p602012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	S65720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
STI-12012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	S65720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	Caco2
STI-12012-06-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	S65720	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Chaperone sampler kit (BD Transduction Lab)
	MDCK
STI-12012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	S65720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	Caco2細胞
STI-12012-11-13		WB	IF	IHC	IP
	BD Transduction		x100
	S65720	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Syntaxin 42011-08-10		WB	IF	IHC	IP
	Sigma		x100
	S9924	3%PFA (in PBS), methanol
	rabbit

	WB, マウス小腸共
SGLT2013-07-04		WB	IF	IHC	IP
	santa cruz		x100
	sc-20581	3%PFA (in PBS)
	goat	0.05%saponin

	Caco2

【s】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
SGLT2013-12-04		WB	IF	IHC	IP
	santa cruz		x100
	sc-20581	3%PFA (in PBS)
	goat	0.05%saponin

	MDCK

【S】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
SV22011-08-12		WB	IF	IHC	IP
	DSHB
	SV2
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

SV22011-08-10		WB	IF	IHC	IP
	DSHB
	SV2
	mouse

	？
SV22012-08-03		WB	IF	IHC	IP
	DSHB		x100
	SV2	3%PFA in PBS
	mouse	0.05% saponin

	PC12
Sec62011-08-14		WB	IF	IHC	IP
	Stressgen
	VAM-SV021
	mouse
	m

SNAP23A2011-08-14		WB	IF	IHC	IP
	Stressgen		x100
	VAP-SV013
	rabbit
	p

【T】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
TGN462011-08-29		WB	IF	IHC	IP
	Cosmo bio		x200

	sheep	【PFA+Triton】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Poly	2ndはDonkey anti-sheep Cy3を使用、MDCKIIダメ Use Cy3 labeled donkey anti-sheep as 2nd Ab. Not suitable for MDCKII.
	Hela細胞
TfnR2011-08-29	H68.4	WB	IF	IHC	IP
	Zymed	x1000-	x5000-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS　37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	Human OK, MDCKII OK, OverexpressionしているArfraptin2を認識する。 Recognize overexpressed Arfaptin2.
	Hela細胞
TIP472011-08-29		WB	IF	IHC	IP
	Progen	x1000	x200

	guinea pig	【PFA+Saponin】:○,【PFA_70%EtOH】:○,【PFA_digitonin】:○【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS【digitonin条件】50ug/ml digitonin /PBS
	Poly
	Hela細胞
tsg1012011-08-29	4A10	WB	IF	IHC	IP
	GeneTex	x1000	x200

	mouse	【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【saponin条件】0.05%　saponin /PBS
	Mono, IgG1	IF:saponin permeabilizeでendo Tsg101見える Endogenous Tsg101 can be recognized by saponin perm.
	Hela細胞
tsg101N2011-08-29	AP2155a	WB	IF	IHC	IP
	ABG

	Rabbit
	Poly
	Hela細胞
TfR2011-09-27	H68.4	WB	IF	IHC	IP
	Zymed	1:1000	1:100
	13-6800
	mouse
	m
	HeLa, A549, MCF7他
human Transferrin Receptor2011-08-12		WB	IF	IHC	IP
	Zymed
	136800
	mouse
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

human Transferrin Receptor2011-08-10		WB	IF	IHC	IP
	Zymed		x100
	136800	3%PFA (in PBS), methanol
	mouse

	マウス小腸
human Transferrin Receptor2011-08-10		WB	IF	IHC	IP
	Zymed		x100
	136800	3%PFA (in PBS), methanol
	mouse
		MDCKは染まる　３Ｔ３は染まらない Stains MDCK, but not 3T3.
	マウス小腸
human Transferrin Receptor2012-08-03		WB	IF	IHC	IP
	Zymed		x100
	136800	3%PFA in PBS
	mouse	0.05% saponin

	PC12
Biotin mouse CD712011-08-10	TFR	WB	IF	IHC	IP
	BD Pharmingen		x100
	557416	3%PFA (in PBS), methanol
	rat
	m
	マウス小腸
Biotin mouse CD712011-08-10	TFR	WB	IF	IHC	IP
	BD Pharmingen		x100
	557416	3%PFA (in PBS), methanol
	rat
	m
	マウス小腸
TfR2011-09-27		WB	IF	IHC	IP
	BD Biosciences	1:1000	1:100
	612125
	mouse
	m	human専用
	HeLa, A549, MCF7他
CD712011-08-10	TFR	WB	IF	IHC	IP
	Zymed		x100
	77103	3%PFA (in PBS), methanol


	マウス小腸
CD712011-08-10	TFR	WB	IF	IHC	IP
	Zymed		x100
	77103	3%PFA (in PBS), methanol


	マウス小腸
Tfn (HRP)2011-08-29 About Product	Monoclonal	WB	IF	IHC	IP
	Abcam
	ab74415
	mouse
	IgG1	x24000 For ELISA
	Hela細胞
rat TGN382011-08-12		WB	IF	IHC	IP
	Serotec
	AHP499G
	sheep
		PC12細胞の免疫蛍光染色（3%PFA固定、0.05%saponinで膜のpermeabilize）よく染まった Good staining for IF of PC12 with 3%PFA, 0.05%saponin

rat TGN382011-08-10		WB	IF	IHC	IP
	Serotec		x100
	AHP499G	3%PFA (in PBS), methanol
	sheep
		メタノール固定で染まるが形がよくない Visualized by methanol fixation, but the TGN is not well preserved.
	マウス小腸
rat TGN382012-08-03		WB	IF	IHC	IP
	Serotec		x100
	AHP499G	3%PFA in PBS
	sheep	0.05% saponin

	PC12
tubulin2011-10-06		WB	IF	IHC	IP
	Cytoskeleton		x400
	ATN02
	Sheep	4%PFA+0.1%Saponin :○
	polyclonal
	HeLa
tubulin2011-10-28		WB	IF	IHC	IP
	Cytoskeleton		x400
	ATN02	4%PFA+0.1%Saponin :○
	Sheep
	polyclonal
	HeLa
TfnR（CD71）2011-08-29	CD71	WB	IF	IHC	IP
	SIGMA		x200-
	C2063
	mouse	【PFA+Triton】:◎【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS
	Mono, IgG1	Lumen側を認識する抗体 Ab recognize luminal part.
	Hela細胞
gamma-tubulin2011-10-06	H-183	WB	IF	IHC	IP
	SantaCruz		x400
	sc-10732
	Rabbit	Methanol: ○
	polyclonal
	HeLa
CD712011-08-10	R17217 TFR	WB	IF	IHC	IP
	Santa Cruz		x100
	sc52504	3%PFA (in PBS), methanol
	rat
	m
	マウス小腸
CD712011-08-10	R17217 TFR	WB	IF	IHC	IP
	Santa Cruz		x100
	sc52504	3%PFA (in PBS), methanol
	rat
	m
	マウス小腸
Thioredoxin2011-08-29		WB	IF	IHC	IP
	SIGMA	x5000
	T0803
	rabbit
	Poly
	Hela細胞

【U】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
human Unc18-1/22011-08-10	N-19	WB	IF	IHC	IP
	Santa Cruz	x1000
	sc14560
	goat
	p
	マウス
human Unc18-22011-08-10	C-20	WB	IF	IHC	IP
	Santa Cruz	x1000
	sc14563
	goat
	p
	マウス

【V】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
VAMP42011-08-29		WB	IF	IHC	IP
	ABR		x500-

	rabbit	【PFA+Triton】:×,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Poly
	Hela細胞
VAMP82011-08-29		WB	IF	IHC	IP
	SySy

	rabbit
	Poly
	Hela細胞
Vti1a2011-08-29	clone 45	WB	IF	IHC	IP
	BD Transduction		x200-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS　37℃10min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
Vti1a2011-08-29		WB	IF	IHC	IP
	SySy

	rabbit
	Poly
	Hela細胞
Vti1b2011-08-29	clone 7	WB	IF	IHC	IP
	BD Transduction		x100-

	mouse	【PFA+Triton】:○,【PFA+Saponin】:○,【MeOH】:◎,【MeOH】:◎【PFA条件】3%PFA/PBS　37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1%　Triton-X100/PBS【saponin条件】0.05%　saponin /PBS
	Mono, IgG1
	Hela細胞
Vti1b2011-08-29		WB	IF	IHC	IP
	SySy

	rabbit
	Poly
	Hela細胞
VAMP3 (cellubrevin)2015-06-02		WB	IF	IHC	IP
	synaptic systems	x1000	x100
	104102	3%PFA (in PBS)
	rabbit
		For WB, 20ug of neural progenitor cell lysate was used
	mouse primary neuron, neural progenitor cell
synaptobrevin/VAMP 22012-08-03		WB	IF	IHC	IP
	Synaptic Sy		x100
	104211	3%PFA in PBS
	mouse	0.05% saponin

	PC12
Synaptobrevin / VAMP22012-11-13	69.1 monoclonal	WB	IF	IHC	IP
	Synaptic Systems	x1000	x100
	104211	3%PFA (in PB)
	mouse
	IgG1	β細胞のinsulin顆粒は強く染まり、acinar細胞のzymogen顆粒は弱い Insulin granules are strongly stained while zymogen granules are weakly stained.
	マウス膵臓
rabbitαVAMP82011-08-10		WB	IF	IHC	IP
	SYSY	x1000
	104302
	rabbit
	p
	マウス
Endobrevin / VAMP82012-11-13		WB	IF	IHC	IP
	Synaptic Systems	x1000	x100
	104302	3%PFA (in PB)
	Rabbit
		acinar細胞のzymogen顆粒が強く染まる。WBはケラチンのノンスペが多い Zymogen granules are strongly stained in IF, but nonspecific keratin staining is evident in WB.
	マウス膵臓

【v】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
VAMP42016-09-08		WB	IF	IHC	IP
	Synaptic systems	1/1000	1/500
	136002	4% PFA
	Rabbit
	IgG
	HeLa, PC12

【V】

Antigen last update	Clone	Application outlook for label
	Maker	Dilution rate
	Catalog code	Fixation condition for IF and IHC
	Host	Fixation and permeabilization condition
	Isotype	Comments
	Samples	Reference
mVAMP72011-08-10	0H307	WB	IF	IHC	IP
	IBL
	2930
	rabbit


Vps102011-08-31	IgG2a	WB	IF	IHC	IP
	Invitrogen (Molecular Probes)
	A-21274
	Mouse
	monoclonal

Vma2 (V-ATPase B subunit)2011-08-31	IgG1	WB	IF	IHC	IP
	Invitrogen (Molecular Probes)
	A-6427	Roberts et al. (1991) Methods Enzymol. Vol 194, 644-661
	Mouse
	monoclonal

TI-VAMP protein2011-08-10	VAMP 7	WB	IF	IHC	IP
	Novus B		x100
	NB1002325	3%PFA (in PBS), methanol
	mouse
	m
	マウス小腸
TI-VAMP protein2011-08-10	VAMP 7	WB	IF	IHC	IP
	Novus B		x100
	NB1002325	3%PFA (in PBS), methanol
	mouse
	m
	マウス小腸
VAMP42011-08-10		WB	IF	IHC	IP
	affinity bioreagents
	pa1-768
	rabbit
	p

VAMP72011-08-10		WB	IF	IHC	IP
	Abnova		x100
	PAB5936	3%PFA (in PBS), methanol
	rabbit
	p
	マウス小腸
Vti1a2012-01-25		WB	IF	IHC	IP
	BD Transduction		x100
	V85620	3%PFA (in PBS)
	mouse	0.05%saponin
	IgG1
	MDCK細胞
Vti1a2012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	V85620	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	Caco2
Vti1a2012-05-21		WB	IF	IHC	IP
	BD Transduction Lab		x100
	V85620	3%PFA in PBS
	mouse	0.05% saponin
	IgG1	Ab in Golgi sampler kit (BD Transduction Lab)
	MDCK

Antibody

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研究機関専門ホームページ制作

一般社団法人日本細胞生物学会Japan Society for Cell Biology

Antibody（organelles, intracellular traffic）

【A】

【a】

【A】

【a】

【A】

【a】

【A】

【a】

【A】

【a】

【A】

【a】

【A】

【B】

【b】

【B】

【C】

【c】

【C】

【D】

【d】

【D】

【E】

【e】

【E】

【e】

【E】

【e】

【E】

【F】

【G】

【g】

【G】

【g】

【G】

【g】

【G】

【g】

【G】

【g】

【H】

【K】

【L】

【M】

【m】

【M】

【N】

【n】

【N】

【O】

【P】

【p】

【P】

【p】

【P】

【p】

【P】

【p】

【P】

【p】

【P】

【p】

【P】

【p】

【R】

【r】

【R】

【r】

【R】

【S】

【s】

【S】

【s】

【S】

【s】

【S】

【s】

【S】

一般社団法人
日本細胞生物学会Japan Society for Cell Biology